Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Alexandra Franco-Garcia; Tilly A Varughese; Yeon Joo Lee; Genovefa Papanicolaou; Marc K Rosenblum; Travis J Hollmann; Guenther Koehne; Farid Boulad; N Esther Babady; Yi-Wei Tang; Susan K Seo

doi:10.1093/ofid/ofx140

Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Alexandra Franco-Garcia, Tilly A Varughese, Yeon Joo Lee, Genovefa Papanicolaou, Marc K Rosenblum, Travis J Hollmann, Guenther Koehne, Farid Boulad, N Esther Babady, Yi-Wei Tang, Susan K Seo

Miami Cancer Institute

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Original language	American English
Journal	Open Forum Infectious Diseases
Volume	4
DOIs	https://doi.org/10.1093/ofid/ofx140
State	Published - Jul 1 2017

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Medicine and Health Sciences
Molecular Biology

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Franco-Garcia, A., Varughese, T. A., Lee, Y. J., Papanicolaou, G., Rosenblum, M. K., Hollmann, T. J., Koehne, G., Boulad, F., Babady, N. E., Tang, Y.-W., & Seo, S. K. (2017). Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Open Forum Infectious Diseases, 4. https://doi.org/10.1093/ofid/ofx140

Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. / Franco-Garcia, Alexandra; Varughese, Tilly A; Lee, Yeon Joo et al.
In: Open Forum Infectious Diseases, Vol. 4, 01.07.2017.

Research output: Contribution to journal › Article › peer-review

Franco-Garcia, A, Varughese, TA, Lee, YJ, Papanicolaou, G, Rosenblum, MK, Hollmann, TJ, Koehne, G, Boulad, F, Babady, NE, Tang, Y-W & Seo, SK 2017, 'Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry', Open Forum Infectious Diseases, vol. 4. https://doi.org/10.1093/ofid/ofx140

Franco-Garcia A, Varughese TA, Lee YJ, Papanicolaou G, Rosenblum MK, Hollmann TJ et al. Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Open Forum Infectious Diseases. 2017 Jul 1;4. doi: 10.1093/ofid/ofx140

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title = "Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry",

author = "Alexandra Franco-Garcia and Varughese, \{Tilly A\} and Lee, \{Yeon Joo\} and Genovefa Papanicolaou and Rosenblum, \{Marc K\} and Hollmann, \{Travis J\} and Guenther Koehne and Farid Boulad and Babady, \{N Esther\} and Yi-Wei Tang and Seo, \{Susan K\}",

note = "Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.",

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T1 - Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

AU - Franco-Garcia, Alexandra

AU - Varughese, Tilly A

AU - Lee, Yeon Joo

AU - Papanicolaou, Genovefa

AU - Rosenblum, Marc K

AU - Hollmann, Travis J

AU - Koehne, Guenther

AU - Boulad, Farid

AU - Babady, N Esther

AU - Tang, Yi-Wei

AU - Seo, Susan K

N1 - Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

PY - 2017/7/1

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DO - 10.1093/ofid/ofx140

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Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

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